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Platinum™ Taq DNA Polymerase DFS Item

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

Manufacturer: life technologies  10966083

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Catalog No. 10966083


Description & Specifications


Includes 1000μL Platinum Taq DNA Polymerase; 50mL 10X PCR Buffer (no MgCl2); 10mL 50mM MgCl2; 10mL KB Extender
No. of Reactions 5000.0
Overhang 3'-A
Polymerase Platinum™ Taq DNA Polymerase
Quantity 5000 reactions
Size (Final Product) 10 kb or less
Storage Requirements Store at -30° to -10°C. Do not store in a frost-free freezer.

Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

  • Convenience: assemble PCR reactions at room temperature
  • Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
  • Product size: use for PCR products up to 10kb
  • Flexibility: optional KB Extender gives more versatility in PCR assays

Using PlatinumTaq DNA Polymerase

  • Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity)
  • PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR