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Platinum™ Taq DNA Polymerase High Fidelity DFS Item


Ideal for amplification of DNA fragments when high yields and robust amplification are required

Manufacturer: life technologies  11304029

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Catalog No. 11304029

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Description & Specifications

Specifications

Includes Platinum™ Taq DNA Polymerase High Fidelity (20μL, at 5U/μL) is supplied with a vial of 10X High Fidelity Buffer [600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4] (1.25mL), and a vial of 50mM MgSO4 (1mL).
Quantity 500 reactions
Storage Requirements Store at -30° to -10°C

High fidelity is provided by a mixture of Platinum Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum automatic “hot-start” technology.

  • Fidelity: greater than six times higher fidelity than Taq DNA polymerase
  • Amplicon size: amplification of fragments up to 15 kb
  • Convenience: room temperature reaction assembly
  • Applications: amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR
Unit definition
  • One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), High Fidelity PCR, Hot Start PCR, Long Fragment PCR, Methylation Analysis, PCR, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Sanger Sequencing, Sanger Sequencing Technology & Accessories, Sequencing