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Platinum™ Pfx DNA Polymerase DFS Item


Built-in hot start

Manufacturer: life technologies  11708039

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Catalog No. 11708039

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Description & Specifications

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
Gel Compatibility Gel Compatibility: Medium
Includes Platinum Pfx DNA Polymerase (500 units total, at 2.5U/μL) is a supplied with a vial (3 x 1mL) of 10X PCRx Enhancer solution, a vial (3 x 1mL) of 10X Pfx amplification buffer, and a vial (1mL) of 50mM MgSO4.Designed for 500 reactions (at 1 unit each in a 50μL reaction volume).
Quantity 500 reactions
Storage Requirements Store at -20°C.

PlatinumPfxDNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumantibody technology provides a simple, automatic, hot-start method that improves PCR specificity. PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of PlatinumPfxDNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

  • 26 times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 12 kb
  • Room temperature reaction assembly
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.
Unit definition
One unit incorporates 10nmoles of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research