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Invitrogen™ Platinum™ Pfx DNA Polymerase DFS Item

Built-in hot start

$210.00 - $808.50

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
Gel Compatibility Gel Compatibility: Medium
Storage Requirements Store at -20°C.
View More Specs
Includes:  Platinum Pfx DNA Polymerase (100 units total, at 2.5U/μL) is a supplied with a vial (1mL) of 10X PCRx Enhancer solution, a vial (1mL) of 10X Pfx amplification buffer, and a vial (1mL) of 50mM MgSO4. Designed for 100 reactions (at 1 unit each in a 50μL reaction volume).

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

11708039

DFS Item
 
life technologies
11708039
500 reactions Each for $808.50

11708013

DFS Item
 
life technologies
11708013
100 reactions Each for $210.00

11708021

DFS Item
 
life technologies
11708021
250 reactions Each for $497.70
Description & Specifications

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
Gel Compatibility Gel Compatibility: Medium
Storage Requirements Store at -20°C.

PlatinumPfxDNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumantibody technology provides a simple, automatic, hot-start method that improves PCR specificity. PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of PlatinumPfxDNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

  • 26 times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 12 kb
  • Room temperature reaction assembly
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.
Unit definition
One unit incorporates 10nmoles of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Approved for shipment on Wet or Dry Ice