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PCR SuperMix DFS Item

Ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification

Manufacturer: life technologies  10572063

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Catalog No. 10572063


Description & Specifications


Exonuclease Activity 5' - 3'
Fidelity 1 X
For Use With (Application) Efficient PCR amplifications
Format SuperMix
GC-Rich PCR Performance Low
Includes Contains 4 bottles (56.25mL each) of PCR SuperMix. Components: 22mM Tris-HCl (pH 8.4), 55mM KCl, 1.65mM Magnesium Chloride, 220μM dGTP, 220μM dATP, 220μM dTTP, 220μM dCTP, 22U/mL recombinant Taq DNA Polymerase, and stabilizers
No. of Reactions 5000.0
Overhang 3'-A
Polymerase Taq DNA Polymerase
Product Type PCR SuperMix
Quantity 5000 reactions
Size (Final Product) 5 kb or less

Simply add template and primers, reducing set-up time by half. PCR SuperMix contains Mg2+, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of fragments up to 5kb. PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions.

ChIP-on-Chip, Chromatin Biology, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR