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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Manufacturer:  Invitrogen™ LSK480001

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Catalog No. K480001



For Research Use Only. Not for use in diagnostic procedures.

Specifications Description & Specifications


Constitutive Expression
20 reactions
Box 1: store at -20°C. Box 2: store at -80°C
Box 1: 20μL pcDNA3.1/V5-His-TOPO (10ng/μL), 100μL 10X PCR Buffer, 10μL dNTP Mix (50mM); 50μL Salt Solution; 1mL Sterile Water; 10μL Control PCR Template (50ng/μL); 10μL Control PCR Primers (100ng/μL each); 20μL T7 Sequencing Primer (100ng/μL); 20μL BGH Reverse Sequencing Primer (100ng/μL); 10μL Expression Control Plasmid (500ng/μL); Box 2: 21 x 50μL TOP10 E. coli cells; 50μL pUC19 Control DNA (10pg/μL); 6mL S.O.C. Medium
Geneticin™ (G-418)
TOPO-TA Vectors, pcDNA
His Tag (6X), V5 Epitope Tag