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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item


Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Manufacturer: invitrogen™  K480040

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Catalog No. K480040

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Description & Specifications

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Functions Available Constitutive Expression
Includes 2 x Box 1: 20μL pcDNA3.1/V5-His-TOPO (10ng/μL); 100μL 10X PCR Buffer; 10μL dNTP Mix (50mM); 50μL Salt Solution; 1mL Sterile Water; 10μL Control PCR Template (50ng/μL); 10μL Control PCR Primers (100ng/μL each); 20μL T7 Sequencing Primer (100ng/μL); 20μL BGH Reverse Sequencing Primer (100ng/μL); 10μL Expression Control Plasmid (500ng/μL). 2 x Box 2: 21 x 50μL TOP10 E. coli cells; 50μL pUC19 Control DNA (10pg/μL); 6mL S.O.C. Medium
Promoter CMV
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Quantity 40 reactions
Vector TOPO-TA Vectors, pcDNA
Storage Requirements Box 1: store at -20°C. Box 2: store at -80°C
Protein Tag His Tag (6X), V5 Epitope Tag

  • No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
  • Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
  • pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
  • C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody • Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
  • Vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis