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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Manufacturer:  Invitrogen™ LSK480001SC

 View more versions of this product

Catalog No. K480001SC


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications Description & Specifications

Specifications

Constitutive
Constitutive Expression
CMV
20 reactions
Store TOPO box at -20°C. TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control: Store at -80°C. Guaranteed stable for 6 months when properly stored.
Transfection
Each pcDNA3.1 Directional TOPO™ Expression Kit contains two boxes. The TOPO box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid.The box contains single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control.
Geneticin™ (G-418)
pcDNA
His Tag (6X), V5 Epitope Tag