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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item


Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Manufacturer: invitrogen™  LSK480001SC

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. K480001SC

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Description & Specifications

Specifications

Includes Each pcDNA3.1 Directional TOPO™ Expression Kit contains two boxes. The TOPO box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid.The box contains single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control.
Constitutive or Inducible System Constitutive
Promoter CMV
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Quantity 20 reactions
Vector pcDNA
Storage Requirements Store TOPO box at -20°C. TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control: Store at -80°C. Guaranteed stable for 6 months when properly stored.
Protein Tag His Tag (6X), V5 Epitope Tag
Delivery Type Transfection
Functions Available Constitutive Expression