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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item


Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Manufacturer: invitrogen™  LSK480001SC

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. K480001SC

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Description & Specifications

Specifications

Includes Each pcDNA3.1 Directional TOPO™ Expression Kit contains two boxes. The TOPO box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid.The box contains single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control.
Storage Requirements Store TOPO box at -20°C. TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control: Store at -80°C. Guaranteed stable for 6 months when properly stored.
Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Functions Available Constitutive Expression
Quantity 20 reactions
Vector pcDNA
Protein Tag His Tag (6X), V5 Epitope Tag
Selection Agent (Eukaryotic) Geneticin™ (G-418)