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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit DFS Item


Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Manufacturer: invitrogen™  K480001SC

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Catalog No. K480001SC

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Description & Specifications

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Functions Available Constitutive Expression
Includes Each pcDNA3.1 Directional TOPO™ Expression Kit contains two boxes. The TOPO box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid.The box contains single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control.
Promoter CMV
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Quantity 20 reactions
Vector pcDNA
Storage Requirements Store TOPO box at -20°C. TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control: Store at -80°C. Guaranteed stable for 6 months when properly stored.
Protein Tag His Tag (6X), V5 Epitope Tag

  • No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
  • Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
  • pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
  • C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody • Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
  • Vector features strong CMV promoter for high-level, constitutive expression
  • C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis