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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit DFS Item

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

Manufacturer: invitrogen™  K490040

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Catalog No. K490040


Description & Specifications


Constitutive or Inducible System Constitutive
Delivery Type Transfection
Functions Available Constitutive Expression
Includes 2 x Box 1: 20μL pcDNA3.1D/V5-His-TOPO (15-20ng/μL); 10μL dNTP Mix; 50μL Salt Solution; 1mL Sterile Water; 20μL T7 Sequencing Primer (0.1μg/μL); 20μL BGH Reverse Sequencing Primer (0.1μg/μL); 10μL Control PCR Template (0.1μg/μL); 10μL Control PCR Primers (0.1μg/μL each); 10μL Expression Plasmid (0.5μg/μL). 2 x Box 2: 6mL S.O.C. Medium; 21 x 50μL TOP10 Cells; 50μL pUC19 Control DNA
Promoter CMV
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Quantity 40 reactions
Vector Directional TOPO Vectors, pcDNA
Storage Requirements Box 1: Store at -20°C); Box 2: Store at -80°C; 6mL S.O.C. Medium may be stored at 4°C or room temperature
Protein Tag His Tag (6X), V5 Epitope Tag

  • Proofreading enzyme is used: fewer errors in cloned genes
  • Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
  • CMV promoter: high expression levels
  • C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
  • 6xHis tag for rapid protein purification on nickel-chelating resin

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis