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Invitrogen™ pBAD TOPO™ TA Expression Kit DFS Item


Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

Manufacturer: invitrogen™  LSK430001

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. K430001

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Description & Specifications

Specifications

Vector pBAD
Inducing Agent Arabinose
Storage Requirements The TOPO box should be stored at -20°C. Store cells at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.
Selection Agent (Eukaryotic) None
Protein Tag His Tag (6X), V5 Epitope Tag
Constitutive or Inducible System Inducible
Includes Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. the box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Promoter araBAD
Quantity 20 reactions