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Invitrogen™ pBAD TOPO™ TA Expression Kit DFS Item


Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

Manufacturer: invitrogen™  K430001

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Catalog No. K430001

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Description & Specifications

Specifications

Constitutive or Inducible System Inducible
Inducing Agent Arabinose
Includes Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. the box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Promoter araBAD
Selection Agent (Eukaryotic) None
Quantity 20 reactions
Vector pBAD
Storage Requirements The TOPO box should be stored at -20°C. Store cells at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.
Protein Tag His Tag (6X), V5 Epitope Tag

  • Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
  • araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
  • C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
  • Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis