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Invitrogen™ pBAD TOPO™ TA Expression Kit DFS Item

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

Manufacturer:  Invitrogen™ LSK430001

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Catalog No. K430001


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications Description & Specifications

Specifications

Inducible
Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. the box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
None
pBAD
His Tag (6X), V5 Epitope Tag
Arabinose
araBAD
20 reactions
The TOPO box should be stored at -20°C. Store cells at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.
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