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Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli DFS Item


Ideal for high-efficiency cloning and plasmid propagation

Manufacturer: invitrogen™  C303003

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Catalog No. C303003

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Description & Specifications

Specifications

Cell Type Chemically Competent
Bacterial Content TOP10F´
Contains One Shot TOP10F′ Chemically Competent E. coli: 21 vials, 50μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 1 bottle, 6mL
Contains F' Episome Yes
Efficiency > 1x109
T1 Phage Resistant No
Improves Plasmid Quality Yes
Includes Blue/White Screening Yes
Recombinant Yes
Species E. coli
For Use With (Application) Cloning, Transformation
Quantity 20 x 50μL
Storage Requirements Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

One Shot TOP10F Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome. TOP10 competent cells are provided at a transformation efficiency of 1 x 109 cfu/μg supercoiled DNA.<br><br>Using One Shot TOP10F' Chemically Competent E. coli<br>The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F cells require IPTG induction for blue/white color screening. High efficiency (> 109 cfu/μg).

Not high-throughput compatible (Manual)

Cloning, Transformation