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One Shot™ TOP10 Chemically Competent E. coli, High Copy Plasmid DFS Item

Ideal for high-efficiency cloning and plasmid propagation

Manufacturer: life technologies  C404006

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Catalog No. C404006

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Description & Specifications


Cell Type Chemically Competent
Bacterial Content TOP10
Contains One Shot TOP10 Chemically Competent E. coli: 42 vials, 50μL each, pUC19 DNA (10pg/μL): 2 vials, 50μL each, S.O.C. medium: 2 bottles, 6mL each
Contains F' Episome No
Efficiency > 1x109
T1 Phage Resistant No
Improves Plasmid Quality Yes
Includes Blue/White Screening Yes
Recombinant Yes
Species E. coli
For Use With (Application) Cloning, Transformation
Quantity 40 reactions
Storage Requirements Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

One Shot TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many Life Technologies cloning kits.

  • Maximization of cloning efficiency in a single-tube format
  • Enhanced genomic DNA cloning capabilities

Not high-throughput compatible (Manual)

Cloning, Transformation