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One Shot™ BL21(DE3)pLysS Chemically Competent E. coli DFS Item


Ideal for use with T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET)

Manufacturer: life technologies  C606010

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Catalog No. C606010

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Description & Specifications

Specifications

Cell Type Chemically Competent
Bacterial Content BL21(DE3), BL21(DE3)pLysS
Contains F' Episome No
Efficiency > 1x108
T1 Phage Resistant No
Improves Plasmid Quality No
Includes Blue/White Screening No
Recombinant No
Species E. coli
For Use With (Application) Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Quantity 10 x 50μL

BL21(DE3)pLysS cells carry both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme, which reduces basal expression of recombinant genes by inhibiting basal levels of T7 RNA polymerase.

Not high-throughput compatible (Manual)

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis