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MultiShot™ StripWell TOP10 Chemically Competent E. coli DFS Item


Allows rapid transformation in a convenient 96-well format

Manufacturer: life technologies  C409610

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Catalog No. C409610

 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Description & Specifications

Specifications

Cell Type Chemically Competent
Bacterial Content TOP10
Contains MultiShot StripWell TOP10 Chemically Competent E. coli: Ten 96-stripwell plates, 50μL per well, pUC19 DNA (10pg/μL): 2 vials, 50μL each, S.O.C. medium: 20 bottles, 15mL each
Contains F' Episome No
Efficiency > 1x108
T1 Phage Resistant No
Improves Plasmid Quality Yes
Includes Blue/White Screening Yes
Recombinant Yes
Species E. coli
For Use With (Application) Cloning, Transformation
Quantity 10 plates
Storage Requirements Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

MultiShot StripWell TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/μg with control plasmid DNA. MultiShot StripWell TOP10 Chemically Competent cells are packaged in a 96-stripwell plate (50μL aliquots) to simplify medium- and high-throughput bacterial transformation.

  • Flexible- designed for medium- to high-throughput cloning
  • Compatible with genomic DNA- the mcrA mutation supports efficient transfection of methylated DNA
  • Convenient —the lacZΔM15 allele permits rapid blue/white screening of transfectants on plates containing X-Gal or Bluo-Gal
  • Efficient—the endA1 mutation reduces endonuclease activity, improving plasmid DNA yields
  • Reliable—the recA1 mutation reduces unwanted recombination

For other high-throughput transformation options, choose MultiShot formatted comp cells or contact your Customer Service Respresentative for custom formatting options.

Cloning, Transformation