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MultiShot™ StripWell TOP10 Chemically Competent E. coli DFS Item


Allows rapid transformation in a convenient 96-well format

Manufacturer: life technologies  C409605

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Catalog No. C409605

 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Description & Specifications

Specifications

Cell Type Chemically Competent
Bacterial Content TOP10
Contains MultiShot StripWell TOP10 Chemically Competent Cell kits include 96-well stripwell plates, 2 x 15mL S.O.C. medium, and pUC19 control plasmid. MultiShot Chemically Competent Cell kits include five, 96-well microtiter plates, 5 x 15mL S.O.C. medium, and pUC19 control plasmid.
Contains F' Episome No
Efficiency > 1x108
T1 Phage Resistant No
Improves Plasmid Quality Yes
Includes Blue/White Screening Yes
Recombinant Yes
Species E. coli
For Use With (Application) Cloning, Transformation
Quantity 5 plates
Storage Requirements Store all cells at -80°C. Store S.O.C. at +4°C. Store plasmid at -20°C.

MultiShot StripWell TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/μg with control plasmid DNA. MultiShot StripWell TOP10 Chemically Competent cells are packaged in a 96-stripwell plate (50μL aliquots) to simplify medium- and high-throughput bacterial transformation.

  • Flexible- designed for medium- to high-throughput cloning
  • Compatible with genomic DNA- the mcrA mutation supports efficient transfection of methylated DNA
  • Convenient —the lacZΔM15 allele permits rapid blue/white screening of transfectants on plates containing X-Gal or Bluo-Gal
  • Efficient—the endA1 mutation reduces endonuclease activity, improving plasmid DNA yields
  • Reliable—the recA1 mutation reduces unwanted recombination

For other high-throughput transformation options, choose MultiShot formatted comp cells or contact your Customer Service Respresentative for custom formatting options.

Cloning, Transformation