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Invitrogen™ Taq DNA Polymerase, native 
A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer
Supplier: Invitrogen™ 18038067
Description
The enzyme consists of single polypeptide with molecular weight of 94 kDa. It has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity.
- Amplification of PCR products up to 5 kb in size
- Enzyme that is licensed and qualified for PCR
- Applications: Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
- Source: Native enzyme is purified from Thermus aquaticus YT1
- Unit definition: One unit of Taq DNA Polymerase is amount of enzyme required to incorporate 10nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C
ChIP-on-Chip, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR
Specifications
| Contains: • 300 μl Taq DNA Polymerase (5 U/μl) • 7.5 ml 10X PCR buffer (without magnesium) • 3 ml Magnesium Chloride (50 mM) Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly. |
|
| Stand-alone enzyme | |
| qPCR, Standard PCR | |
| Standard | |
| DNA Polymerase | |
| 5 U/μL | |
| No | |
| 3'-A | |
| 5 kb or less |
| Primer-probe | |
| Low | |
| Taq DNA Polymerase | |
| 5' - 3' | |
| 3 x 500 Units | |
| 1 X | |
| 1500 | |
| Separate Components | |
| DNA |
For Research Use Only. Not for use in diagnostic procedures.