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Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL) 
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
Supplier: Invitrogen™ 28025021
Description
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT)25 as template-primer.
Features of M-MLV Reverse Transcriptase include:
• Excellent thermostability with optimal activity at 37°C
• Can be used to synthesize first-strand cDNA up to 7 kb
• Multiple applications including synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
Source
Purified from E. coli expressing the pol gene of M-MLV on a plasmid
Performance and quality testing
SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)25, 0.1 mg/mL BSA, and enzyme in 50 μL for 10 min at 37°C
Specifications
• M-mLV RT, 1 mL (200 U/μL) |
|
| 50 min. | |
| 37°C | |
| Yes | |
| 200 U/μL | |
| 1,000 Reactions | |
| Reverse Transcription | |
| RNA |
| Stand-alone Enzyme | |
| Reverse Transcription | |
| M-MLV | |
| Wet Ice | |
| First-Strand cDNA | |
| Separate components | |
| Up to 7 kb |
For Research Use Only. Not for use in diagnostic procedures.