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Taq DNA Polymerase, Recombinant DFS Item


A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Manufacturer: life technologies  10342046

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Catalog No. 10342046

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Description & Specifications

Specifications

Exonuclease Activity 5' - 3'
Fidelity 1 X
Format Separate Components
GC-Rich PCR Performance Low
Includes 3 x 100μL Taq DNA Polymerase (5U/μL, 1500 units total); 7.5mL 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); 3mL Magnesium Chloride (50mM)
Overhang 3'-A
Polymerase Taq DNA Polymerase
Product Type Taq DNA Polymerase, Recombinant
Quantity 3 x 500 units
Size (Final Product) 5 kb or less
Storage Requirements Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly.

The enzyme consists of a single polypeptide with a molecular weight of 94kDa. It has a 5'-3' DNA polymerase activity and a 5'-3' exonuclease activity.

  • Choice of recombinant or native enzyme
  • Amplification of PCR products up to 5kb in size
  • An enzyme that is licensed and qualified for PCR
  • Source: Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli
  • Applications: Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
  • Unit definition: One unit of TaqDNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C