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Elongase™ Enzyme Mix DFS Item


Amplifies both long and short DNA templates

Manufacturer: life technologies  10480028

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Catalog No. 10480028

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Description & Specifications

Specifications

Composition Elongase Enzyme Mix is supplied with 5X buffer A and 5X buffer B. PCR SuperMix High Fidelity is supplied in a 1.1X concentration. It contains Elongase Enzyme Mix, dNTPs, salts, and MgSO4.
Fidelity (vs. Taq) Fidelity (vs. Taq): 5X
Gel Compatibility Gel Compatibility: Low
Quantity 500 reactions
Storage Requirements Store at -20°C. Guaranteed stable for 6 months when properly stored.

The Elongase Amplification System is ideal for amplifying DNA fragments up to 30 kb from purified DNA or crude samples. The enzyme mix is an optimized mixture of recombinant thermostable DNA polymerases, TaqDNA polymerase, and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcus species GB-Dpolymerase.

  • Five-times higher fidelity than Taq DNA polymerase alone
  • A buffer system that allows amplification of simple and complex genomic targets
  • Amplification of long PCR templates or cDNA templates in RT-PCR applications

Long Fragment PCR, PCR, PCR and Real-Time PCR