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Electrocomp™ TOP10 Electrocomp Kit DFS Item

Supplied at transformation efficiency of 1 × 109 cfu/μg supercoiled DNA for high-efficiency cloning and plasmid propagation

Manufacturer: life technologies  C66455

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Catalog No. C66455


Description & Specifications


For Use With (Application) High-efficiency cloning and plasmid propagation
Includes Contains enough electrocompetent cells for 12 x 40μL rxns or 25 x 20μL rxns
Quantity 5 x 100 μL

  • Allows stable replication of high-copy number plasmids
  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA and better results in downstream applications due to elimination of non-specific digestion by Endonuclease I
  • recA1 for reduced occurrence of non-specific recombination in cloned DNA

  • Bacterial/Yeast Strain: TOP10


Cloning, Transformation