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Electrocomp™ One Shot™ One Shot TOP10 Electrocomp E. coli DFS Item


Supplied at transformation efficiency of 1 x 109 cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Manufacturer: life technologies  C404052

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Catalog No. C404052

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Description & Specifications

Specifications

For Use With (Application) High-efficiency cloning and plasmid propagation
Quantity 20 reactions

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
  • recA1 for reduced occurrence of nonspecific recombination in cloned DNA
  • Bacterial/Yeast Strain: TOP10

Cloning, Transformation