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Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit DFS Item

Combines the high performance of Invitrogen Cloned AMV RT with the convenience of optimized, prequalified reagents

$359.10 - $1,199.10

Specifications

For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Storage Requirements Store at -70deg.C
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

12328040

DFS Item
 
life technologies
12328040
100μL cloned AMV RT (15U/μL), 500μL 5X cDNA synthesis buffer, 2 x 250μL 0.1M DTT, 100μL 10mM dNTP mix, 25μL RnaseOUT (40U/μL), 25μL oligo(dT)20 (50μM), 250μL random hexamers (50ng/μL), 1.25mL DEPC-treated water, product profile sheet 100 reactions (375 units) Each for $1,199.10

12328032

DFS Item
 
life technologies
12328032
25μL cloned AMV RT (15U/μL), 500μL 5X cDNA synthesis buffer, 250μL 0.1M DTT, 100μL 10mM dNTP mix, 25μL RnaseOUT (40U/μL), 25μL oligo(dT)20 (50μM), 50μL random hexamers (50ng/μL), 1.25mL DEPC-treated water, product profile sheet 25 reactions (375 units) Each for $359.10
Description & Specifications

Specifications

For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Storage Requirements Store at -70deg.C

The subunits of the avian myeloblastosis virus gene were co-expressed and purified to produce this recombinant AMV RT. Unlike the commonly available native enzyme, Invitrogen Cloned AMV is a high-purity preparation that results in greater specific activity, leading to improved yields and superior sensitivity. To achieve high-quality routine RT-PCR, combine with Platinum Taq DNA Polymerase for high-specificity PCR or with Platinum Taq DNA Polymerase High Fidelity for subsequent PCR cloning.

  • Uses high-purity Cloned AMV for higher yields
  • Includes 5X first-strand buffer with proprietary agents for more robust performance
  • Replaces the cDNA Cycle Kit and AMV Reverse Transcriptase System
  • Detects transcripts in 1.0pg of RNA
  • Detects various mRNA targets ranging in size from 1.6kb to 9.4kb
  • Concentration: 15U/μL

  • Optimal reaction temperature: 45-55°C
  • Final product size: 9.4kb or less

PCR & Real-Time PCR, Reverse Transcription

Shipping Condition: Dry ice