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Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit DFS Item

Combines the high performance of Invitrogen Cloned AMV RT with the convenience of optimized, prequalified reagents

$369.00 - $1,232.00

Specifications

For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Storage Requirements Store at -70deg.C
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



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Catalog Number Mfr. No. Includes Quantity Price Quantity  
Catalog Number Mfr. No. Includes Quantity Price Quantity  
12328040 Life Technologies
LS12328040
100μL cloned AMV RT (15U/μL), 500μL 5X cDNA synthesis buffer, 2 x 250μL 0.1M DTT, 100μL 10mM dNTP mix, 25μL RnaseOUT (40U/μL), 25μL oligo(dT)20 (50μM), 250μL random hexamers (50ng/μL), 1.25mL DEPC-treated water, product profile sheet 100 reactions (375 units) Each for $1,232.00
12328032 Life Technologies
LS12328032
25μL cloned AMV RT (15U/μL), 500μL 5X cDNA synthesis buffer, 250μL 0.1M DTT, 100μL 10mM dNTP mix, 25μL RnaseOUT (40U/μL), 25μL oligo(dT)20 (50μM), 50μL random hexamers (50ng/μL), 1.25mL DEPC-treated water, product profile sheet 25 reactions (375 units) Each for $369.00
Specifications

Description

The subunits of the avian myeloblastosis virus gene were co-expressed and purified to produce this recombinant AMV RT. Unlike the commonly available native enzyme, Invitrogen Cloned AMV is a high-purity preparation that results in greater specific activity, leading to improved yields and superior sensitivity. To achieve high-quality routine RT-PCR, combine with Platinum Taq DNA Polymerase for high-specificity PCR or with Platinum Taq DNA Polymerase High Fidelity for subsequent PCR cloning.

  • Uses high-purity Cloned AMV for higher yields
  • Includes 5X first-strand buffer with proprietary agents for more robust performance
  • Replaces the cDNA Cycle Kit and AMV Reverse Transcriptase System
  • Detects transcripts in 1.0pg of RNA
  • Detects various mRNA targets ranging in size from 1.6kb to 9.4kb
  • Concentration: 15U/μL

  • Optimal reaction temperature: 45-55°C
  • Final product size: 9.4kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Description & Specifications

Specifications

First-Strand cDNA Synthesis, RT-PCR
Store at -70deg.C