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Clonase™ Gateway™ BP Clonase Enzyme Mix DFS Item

Manufacturer: life technologies  11789013

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Catalog No. 11789013

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Description & Specifications


Includes Proteinase K solution (2μg/μL), 30% PEG 8000/30mM MgCl2 Solution and a positive control vector
Quantity 20 reactions
Storage Requirements Store at -80°C; BP Clonase II Enzyme mixtures at -20°C. Guaranteed stable for 6 months when properly stored.

  • Proteins catalyze in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and Donor vector (containing attP sites) to generate Entry clones
  • BP Clonase II enzyme format is single mix of both enzyme and buffer to ensure enzyme stability and enable convenient 10μL reaction set up with fewer pipetting steps, which both maximize recombination efficiency and minimize pippetting
  • Original format of BP Clonase enzyme is stand alone enzyme blend and reaction buffer provided in separate tubes

Cloning, Gateway Cloning, Gateway Recombination