Invitrogen™ Gateway™ BP Clonase™ Enzyme Mix
Manufacturer: Invitrogen™ LS11789013
- Proteins catalyze in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and Donor vector (containing attP sites) to generate Entry clones
- BP Clonase II enzyme format is single mix of both enzyme and buffer to ensure enzyme stability and enable convenient 10μL reaction set up with fewer pipetting steps, which both maximize recombination efficiency and minimize pippetting
- Original format of BP Clonase enzyme is stand alone enzyme blend and reaction buffer provided in separate tubes
Cloning, Gateway Cloning, Gateway Recombination
All Gateway® BP Clonase® enzyme kits include proteinase K solution (2 µg/µl), 30% PEG 8000/30 mM MgCl2 Solution and a positive control vector. Store BP Clonase® enzyme at -80°C; BP Clonase® II Enzyme mixtures at -20°C. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.