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Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase) DFS Item

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

$145.00 - $154.00

Specifications

Quantity 1000 units
Storage Requirements Store at -20°C
View More Specs
Includes:  A vial of 10X dephosphorylation buffer [500mM Tris-HCl (pH 8.5), 1mM EDTA], vial of dilution buffer [50% glycerol, 25mM Tris-HCl (pH 7.6), 1mM MgCl2 , and 0.1mM ZnCl2 ]

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Concentration Price Quantity    

18009027

DFS Item
 
life technologies
LS18009027
1U/μL Each for $145.00

18009019

DFS Item
 
life technologies
LS18009019
20U/μL Each for $154.00
Description & Specifications

Specifications

Quantity 1000 units
Storage Requirements Store at -20°C

  • Concentration: 20 units/μL
  • Source: Purified from calf intestinal mucosa
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
  • Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
  • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900μL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

Shipping Conditions: Approved for shipment on wet or dry ice