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Invitrogen™ ChargeSwitch™ NoSpin Plasmid Micro Kit DFS Item

Optimized for automation

Manufacturer: invitrogen™  CS1020110

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Magnetic accessory

Catalog No. CS1020110


Description & Specifications


For Use With (Application) Cloning, In Vitro Transcription, Next-Generation Sequencing, Nucleic Acid Labeling, PCR, Sequencing, Transfection, Transformation
Isolation Technology ChargeSwitch™ Chemistry
Quantity 960 preps
Sample Size >100 μg (Small-Scale) Plasmid DNA
Sample Type Bacterial Culture
Storage Requirements Store as follows: ChargeSwitch™ Precipitation Buffer (N5) at 4°C. Mix the RNase I in ChargeSwitch™ Resuspension Buffer (R4) and store the resulting solution at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.


ChargeSwitch™ MagnaClear™ Beads
ChargeSwitch™ Magnetic Beads
ChargeSwitch™ Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
ChargeSwitch™ Lysis Buffer (L9)
ChargeSwitch™ Precipitation Buffer (N5)
ChargeSwitch™ Wash Buffer (W11)
ChargeSwitch™ Wash Buffer (W12)
ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
Target Plasmid DNA

  • Sample size 0.5-1mL overnight culture; Typical yield up to 5μg
  • Provides centrifuge-free purification, ideal for automation
  • Avoids introduction of enzymatic contaminants for better results
  • ChargeSwitch NoSpin Kits provide centrifuge-free microprep plasmid purification, ideal for automation
  • Novel NoSpin reagent causes bacterial cell flocculation and allows cells to be pelleted without the need for automation bottlenecks such as centrifugation

DNA & RNA Purification & Analysis, DNA Extraction, Miniprep, Plasmid DNA Purification