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AccuPrime™ SuperMix II DFS Item


Designed for amplification of genomic DNA templates 200bp-4kb

Manufacturer: life technologies  12341012

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Template and primers

Catalog No. 12341012

  • / Each
 

Description & Specifications

Specifications

Composition 40mM Tris-HCl (pH 8.4); 100mM KCl; 3mM MgCl2, 400μM each dGTP, dATP, dTTP, dCTP; AccuPrime Taq DNA Polymerase; thermostable AccuPrime protein; stabilizers
Fidelity (vs. Taq) Fidelity (vs. Taq): 2X
Gel Compatibility Gel Compatibility: Low
Includes AccuPrime SuperMix II is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each are provided. AccuPrime SuperMix II Components: 40mM Tris-HCl (pH 8.4); 100mM KCl; 3mM MgCl2, 400μM each dGTP, dATP, dTTP, dCTP; AccuPrime Taq DNA Polymerase; thermostable AccuPrime protein; stabilizers. 200 rxns: 2 x 1.25mL
Quantity 200 reactions
Storage Requirements Store at -20°C in a non-frost-free freezer.

AccuPrime SuperMix II is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. For accurate PCR performance, AccuPrime SuperMix II sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. In addition to a powerful DNA polymerase, AccuPrime SuperMix I includes Platinumanti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR. This unique combination allows for success, even under previously suboptimal conditions. AccuPrime SuperMix II is designed for amplification of genomic DNA amplicons, plasmid DNA, or cDNA templates up to 4kb in length. Alternatively, AccuPrime SuperMix I is optimized to amplify a tighter range of targets less than 200bp in length.

Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR