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AccuPrime™ SuperMix I DFS Item


Efficient PCR amplification, reduce set-up time by half

Manufacturer: life technologies  12342010

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Catalog No. 12342010

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Description & Specifications

Specifications

Composition 40mM Tris-HCl (pH 8.4), 100mM KCl, 3mM MgCl2, 400μM dGTP, 400μM dATP, 400μM dTTP, 400μM dCTP, AccuPrime Taq DNA Polymerase, thermostable AccuPrime protein, stabilizers
Fidelity (vs. Taq) Fidelity (vs. Taq): 2X
Gel Compatibility Gel Compatibility: Low
Includes AccuPrime SuperMix I is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each are provided. AccuPrime SuperMix I Components: 40mM Tris-HCl (pH 8.4), 100mM KCl, 3mM MgCl2, 400μM dGTP, 400μM dATP, 400μM dTTP, 400μM dCTP, AccuPrime Taq DNA Polymerase, thermostable AccuPrime protein, stabilizers. 200 rxns: 2 x 1.25mL
Quantity 200 reactions
Storage Requirements Store at -20°C in a non-frost-free freezer.

AccuPrime SuperMix I is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. For accurate PCR performance, AccuPrime SuperMix I sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. In addition to a powerful DNA polymerase, AccuPrime SuperMix I includes Platinumanti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR. This unique combination allows for success, even under previously suboptimal conditions. AccuPrime SuperMix I is designed for amplification of genomic DNA amplicons (?200 bp), plasmid DNA, or cDNA templates. Alternatively, AccuPrime SuperMix II is designed to amplify a broader range of amplicon sizes up to 4kb.

  • Built-in hot start

Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR