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AccuPrime™ Taq DNA Polymerase System DFS Item


Suitable for wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications

Manufacturer: life technologies  12339016

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Catalog No. 12339016

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Description & Specifications

Specifications

Exonuclease Activity 5' - 3'
Fidelity 2 X
GC-Rich PCR Performance Low
Includes AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL). 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
No. of Reactions 200.0
Overhang 3'-A
Polymerase AccuPrime™ Taq DNA Polymerase
Size (Final Product) 5 kb or less
Storage Requirements Store at -20°C.

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases. Platinumanti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, providing optimal performance between 55°C and 65°C.

  • Specificity: Control mispriming at every cycle to get single-band PCR products
  • Ease of use: minimize reaction optimization and primer set redesign
  • Convenience: conveniently assemble reactions at room temperature

  • AccuPrime buffers: 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations
  • Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
  • For small genomic DNA amplicons (≤200bp), cDNA, or plasmids

Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, Multiplex PCR, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR