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AccuPrime™ Taq DNA Polymerase, High Fidelity DFS Item


Amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

Manufacturer: life technologies  12346094

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Catalog No. 12346094

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Description & Specifications

Specifications

Includes AccuPrime™ Taq DNA Polymerase High Fidelity (40μL, at 5U/μL)) is supplied with: 50mM MgSO4 (1mL), 10X AccuPrime™ PCR Buffer I (1mL), 10X AccuPrime™ PCR Buffer II (1mL)
Quantity 1000 reactions
Storage Requirements Store at -30° to -10°C

  • High specificity and yield for the most robust PCR amplification
  • 9-fold higher fidelity than TaqDNA polymerase alone
  • Mimimal optimization steps, even with nonoptimized primer sets
  • Efficient amplification of targets over a broad size range up to 20kb
How it work
High fidelity is achieved by a combination of Platinum antiTaqDNA polymerase antibodies that inhibit polymerase activity, providing an automatic “hot-start”, and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcusspecies GB-D. The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Long Fragment PCR, Methylation Analysis, PCR, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research