Please login to your online account to display your discounted pricing

AccuPrime™ Pfx DNA Polymerase DFS Item

Provides higher fidelity than Pfu DNA polymerase

Manufacturer: life technologies  12344024

 View all options for this product

Catalog No. 12344024


Description & Specifications


Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
Gel Compatibility Gel Compatibility: Low
Includes AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.
Quantity 200 reactions
Storage Requirements Store at -20°C.

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.

  • Achieve greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplify fragments up to 12 kb
  • Minimize PCR optimization
  • Built-in hot start

Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research