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AccuPrime™ GC-Rich DNA Polymerase DFS Item

High-specificity, high-yield PCR amplification of GC-rich templates

Manufacturer: life technologies  12337016

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Catalog No. 12337016


Description & Specifications


Fidelity (vs. Taq) Fidelity (vs. Taq): 2X
Gel Compatibility Gel Compatibility: High
Includes AccuPrime GC-Rich DNA Polymerase (100 μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mMMgSO4. Both buffers contain dNTPs, MgSO4, and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).
Quantity 200 reactions
Storage Requirements Store at -20°C.

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

  • Yield: high yields for targets up to 5kb in length
  • Specificity: AccuPrime accessory proteins for improved PCR specificity
  • Sensitivity: sensitivity down to 5ng of template DNA
  • Robust and specific amplifications
  • Built-in hot start

The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition
One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

Amplification of Bisulfite-Treated DNA, GC-Rich PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research