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Thermo Scientific™ Imject™ Mariculture KLH and EDC Conjugation Kit

Prepare immunogens (hapten-carrier conjugates) with your antigen of choice and mariculture KLH using different conjugation chemistries.

$49.08 - $669.90

Specifications

Protein KLH, keyhole limpet hemocyanin
View More Specs
Products
Catalog Number Mfr. No. Description Price Quantity    

PI77159

 
thermo scientific™
PI77159
Imject Purification Buffer Salts Each for $49.08

PI77600

 
thermo scientific™
77600
  • Imject mcKLH (in PBS)
  • Solubility: At a concentration of 10 mg/mL in Milli-Q water, sample is clear to turbid blue solution
Each for $343.35

PI77649

 
thermo scientific™
77649
Imject mcKLH Subunits, High Purity Each for $422.10

PI77653

 
thermo scientific™
77653
Imject mcKLH (in MES buffer) Each for $76.91

PI77671

 
thermo scientific™
77671
Imject EDC mcKLH Spin Kit Each for $669.90
Description & Specifications

Specifications

Protein KLH, keyhole limpet hemocyanin

Thermo Scientific™ Imject Mariculture Keyhole Limpet Hemocyanin (mcKLH) is formulated to achieve optimum performance with different conjugation chemistries, and includes a complete kit for carbodiimide-mediated hapten-carrier preparation.

Mariculture KLH (mcKLH) is purified and lyophilized in buffers that optimize its stability and solubility for hapten conjugation. The large, complex protein contains hundreds of sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers.

Preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking. Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC). The Imject EDC Spin Kit includes ready-to-use units of KLH carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method.

Highlights:

  • Convenient packages – choose lyophilized KLH protein or complete EDC conjugation kit

  • High-yield conjugation – each molecule of KLH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers

  • Validated quality – purified and stabilized mariculture KLH maintains solubility in aqueous solutions, unlike traditional sources of the carrier protein

  • Sustainable source – KLH is harvested from select populations of the mollusk Megathura crenulata (keyhole limpet) that are grown in mariculture, not extracted from wild populations

  • Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin