Thermo Scientific™ Imject™ Maleimide-Activated BSA Spin Kit

Description

Thermo Scientific™ Imject Maleimide-Activated BSA is sulfhydryl-reactive bovine serum albumin for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

Purified, carrier-grade BSA is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Several antigen molecules can be conjugated to each BSA molecule to increase their immunogenicity and likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated BSA protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.

Highlights:

• Convenient packages – choose stabilized, lyophilized Maleimide-Activated BSA or complete immunogen conjugation kit

• Optimized activation – specifications require that each lot is activated with 15 to 20 moles of sulhydryl-reactive maleimide per mole of BSA, ensuring consistently high levels of conjugation

• Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production

• Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals

• High-quality carrier protein – pure BSA (Fraction V) is activated using high-quality Thermo Scientific Sulfo-SMCC crosslinker and formulated to ensure high stability and consistent performance

• Immunogenic and soluble – moderately immunogenic and highly soluble, BSA is useful for preparation of the primary immunogen or as an irrelevant hapten-carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten

Includes:

Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization