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Cytiva RAPD Analysis Beads
For performing DNA-profiling experiments using the randomly amplified polymorphic DNA (RAPD) technique
Supplier: Cytiva 27950201
Includes: RAPD analysis beads, control E. coli BL21 DNA, control E. coli C1a DNA, and RAPD analysis primer 2. (DNA from two E. coli strains and RAPD analysis primer 2 are provided as controls.)
The illustra RAPD Analysis Kit (Mfr. No. 27-9502-01) consists of the Ready-To-Go™ RAPD Analysis Beads and six lyophilized primers which can be used with RAPD Analysis Beads. Each primer in the set is an arbitrary 10-mer which is specifically designed for use in RAPD analysis.
Description
- Preformulated, single-dose, ambient-temperature-stable reaction beads ensure greater reproducibility between reactions, minimize pipetting steps and reduce the potential for pipetting errors and contamination
- RAPD reactions are simple to perform—simply add genomic DNA solution and primer to a tube of RAPD Analysis Beads to a final reaction volume of 25 μL and cycle the reaction
- Each lot of RAPD Analysis Beads is function-tested to ensure its ability to generate a differential banding pattern between the two control E. coli strains using RAPD Analysis Primer 2 included with the RAPD beads
- RAPD reactions are preoptimized for use with a wide variety of organisms
- Beads are provided in thin-walled 0.5 mL tubes compatible with most thermal cyclers
Specifications
| Six primers, room temperature stable bead containing Buffer, dATP, dCTP, dGTP, dTTP, BSA, thermostable DNA Polymerases, control E. coli BL21(DE3) DNA: 1μg of E. coli BL21(DE3) DNA, lyophilized, control E. coli C1a DNA: 1μg of E. coli | |
| 8.3 | |
| 100 Reactions, 6 Primers | |
| 500 μL |
| Tube | |
| Analysis Kit | |
| Standard PCR |