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IL-4 Rat anti-Mouse, PE-Cyanine7, Clone: BVD6-24G2, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS25704282

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Catalog No. 501129572



Description

Description

Description: The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells. Applications Reported: This BVD6-24G2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BVD6-24G2 antibody has been tested intracellular staining and flow cytometric analysis of restimulated mouse splenocytes cultures. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
Specifications

Specifications

IL-4
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P07750
Interleukin-4, BSF1, IL-4, Lymphocyte stimulatory factor 1, MGC79402
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
BVD6-24G2
PE-Cyanine7
Il4
Liquid
Il4
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16189
Documents

For Research Use Only.