IL-31, eFluor 660, clone: 31SNEZE, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS50931942
Interleukin 31, or IL-31 is a cytokine secreted by differentiated CD4+ T helper type 2 (Th2) cells. It is also released during inflammation of the skin, by macrophages and T cells. Because IL-31 is secreted by activated Th2 cells, it has a role in host defense against infection and may be involved in asthma, allergy and different types of bowel diseases.Description: This 31SNEZE monoclonal antibody reacts with human IL-31. Interleukin-31 (IL-31) is a recently-discovered member of the IL-6 family of cytokines. It is expressed by CD4+ T cells, particularly those with a Th2 phenotype, and mast cells. The IL-31 receptor is a heterodimer composed of one IL-31RA and one OSMR beta subunit. This receptor is constitutively expressed by various cells types, including macrophages, monocytes, eosinophils, keratinocytes, and epithelial cells. IL-31 receptor signaling leads to STAT3 activation and promotes the release of proinflammatory cytokines and chemokines, such as IL-1 beta, IL-6, IL-8, CXCL1, and CCL2. Over-expression of IL-31 is observed in patients with allergic asthma, allergic rhinitis, and atopic dermatitis, with a positive correlation between circulating levels and disease severity.Applications Reported: This 31SNEZE antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 31SNEZE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of human peripheral blood lymphocytes. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Flow Cytometry, Flow Cytometry|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.