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IL-12 Mouse anti-Human, eFluor 660, Clone: SNKY35, eBioscience™

Mouse Monoclonal Antibody

$172.55 - $433.55

Specifications

Antigen IL-12
Clone SNKY35
Host Species Mouse
Gene Alias Interleukin-12, Interleukin-12 subunit alpha, IL-12A, IL-12p35
Species Reactivity Human
View More Specs

 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501124456

 
affymetrix
50735941
25 tests Each for $172.55

501124457

 
affymetrix
50735942
100 tests Each for $433.55
Description & Specifications

Specifications

Antigen IL-12
Clone SNKY35
Host Species Mouse
Gene Alias Interleukin-12, Interleukin-12 subunit alpha, IL-12A, IL-12p35
Species Reactivity Human
Applications Flow Cytometry (Intracellular Staining)
Regulatory Status RUO
Conjugate eFluor 660
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 5μL (0.03μg)/test

This SNKY35 monoclonal antibody reacts with human p35, a component of two of the four members of the interleukin (IL)-12 family of cytokines. The IL-12 family members are heterodimeric cytokines composed of an alpha-chain, such as p35, and a beta-chain, such as p40. The alpha-chain proteins are thought to be the limiting factors for secretion of IL-12 family member cytokines, as expression of the alpha-chain is more restricted than that of the beta-chain proteins, and the two subunits may need to be co-expressed in order for cytokine secretion to occur. IL-12 family members are secreted primarily by antigen-presenting cells in response to various TLR agonists and other inflammatory stimuli. The exact types of stimuli present and kinetics of stimulation determine the balance of IL-12 family member production.

The p35 protein was first identified as part of the IL-12 cytokine, which is also known as IL-12p70. Secretion is induced by IFN gamma and agonists of TLR3, 4, or 7 and is inhibited by Th2 cytokines. IL-12 is essential for the differentiation and maintenance of Th1 cells. The other subunit, p40, is expressed in much higher quantities than p35, and may be secreted independently as a monomer or homodimer. Free p40 may act as an antagonist of IL-12 function.

More recently, p35 was also found to dimerize with EBI3 (Epstein-Barr Virus-Induced Gene 3) to form IL-35. The biology of IL-35 is incompletely understood, and it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions. IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naïve CD4+ cells cultured in its presence.

Testing of SNKY35 in the context of IL-35 is currently in process. For additional information, please contact technical support.