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IL-12/IL-23 p40, Alexa Fluor 488, clone: C17.8, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS53712382

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Catalog No. 501124613



Description

Description

IL-23 is a member of the IL-12 cytokine family which is produced by dendritic cells and other antigen presenting cells in the peripheral tissues. This cytokine is a heterodimer composed of an IL-12p40 subunit, and a p19 subunit. IL-23 plays an important role in stimulating memory T-cells. It is also required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells. Th17 cells play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade.

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.Applications Reported: This C17.8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This C17.8 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-12/IL-23 p40
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P43432
IL12B, Il12p40, interleukin 12B, RP23-388G23.1, Il-12b, Il-12p40, p40, IL-12 p40, IL-23 subunit p40, IL12P40
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
C17.8
Alexa Fluor 488
Il12b
Liquid
Il12b
IgG2a, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16160
Documents

For Research Use Only.