IL-10, Biotin, clone: JES5-2A5, eBioscience™
Rat Monoclonal Antibody
Manufacturer: Life Technologies LS13710281
Interleukin-10, originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.6kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production.Description: The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody is a neutralizing antibody. Mouse IL-10 is an 18kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.Applications Reported: The JES5-2A5 antibody has been reported for use in ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.Applications Tested: The JES5-2A5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-10 (IL-10) in combination with the purified JES5-16E3 (14-7101) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Interleukin-10, CSIF, IL-10, IL10A, TGIF|
|ELISA, Dot blot|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze.|
For Research Use Only.