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Rat IgM, PE-eFluor 610, clone: eBRM, Isotype Control, eBioscience™

Rat Isotype Control

Manufacturer:  Life Technologies LS61434180

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Catalog No. 501124816



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal rat IgM K is useful as an isotype control immunoglobulin.Applications Reported: Rat IgM Isotype Control PE-eFluor™ 610 has been reported for use in flow cytometric analysis.Applications Tested: Rat IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody. PE-eFluor™ 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red™). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Rat IgM
Control
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
IgM, IgM Mu
IgM, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eBRM
PE-eFluor 610
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
Documents

For Research Use Only.