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Rat IgG2b kappa, FITC, clone: eB149/10H5, Isotype Control, eBioscience™

Rat Isotype Control

Manufacturer:  Life Technologies LS11403182

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Catalog No. 501129627



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2b, kappa, isotype control has been reported for use as a control for immunofluorescent staining, flow cytometric analysis, immunohistochemistry and immunocytochemistry.Applications Tested: This rat IgG2b, kappa, isotype control has been tested by immunofluorescent staining and flow cytometric analysis of mouse splenocyte suspensions and can be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Rat IgG2b kappa
Control
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG2, IgG2b, IgG2bk
IgG2b, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
eB149/10H5
FITC
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
Documents

For Research Use Only.