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Mouse anti-Rat IgG2a, eFluor 615, Clone: r2a-21B2, Secondary Antibody, eBiocience™

Mouse Monoclonal Secondary Antibody

Manufacturer:  Life Technologies LS42481780

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Catalog No. 501130717



Description

Description

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies.Applications Reported: This R2a-21B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry and immunohistochemistry using a rat primary antibody at 10μg/mL. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Rat IgG2a
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Murine
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
r2a-21B2
eFluor 615
Liquid
IgG1, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Secondary
Rat
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For Research Use Only.