Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™, Invitrogen™

Description

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: PE-Cy7 Rat IgG2a Iso Cntrl has been reported for use in flow cytometric analysis. Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2a kappa Control antibody specifically detects Rat IgG2a kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.