Rat IgG2a kappa, PE, clone: eBR2a, Isotype Control, eBioscience™
Rat Isotype Control
Manufacturer: Life Technologies LS12432142
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: This Rat IgG2a K Isotype Control is offered in 2 formats: - μg size: This has been tested by flow cytometric analysis of mouse splenocytes and should be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis human peripheral blood cells. This can be used at test size: 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Rat IgG2a kappa|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|IgG2, IgG2a, IgG2k|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Control, Flow Cytometry|
For Research Use Only.