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Rat IgG2a kappa, eFluor 660, clone: eBR2a, Isotype Control, eBioscience™

Rat Isotype Control

Manufacturer:  Life Technologies LS50432182

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Catalog No. 501129538



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.Applications Reported: eFluor™ 660 Rat IgG2a Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: eFluor™ 660 Rat IgG2a Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Rat IgG2a kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2k
IgG2a, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
eBR2a
eFluor 660
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
Documents

For Research Use Only.