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Rat IgG2a kappa, Biotin, clone: eBR2a, Isotype Control, eBioscience™

Rat Isotype Control

Manufacturer:  Life Technologies LS13432182

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Catalog No. 501129638



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, flow cytometric analysis, and ELISA.Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Rat IgG2a kappa
Control
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2k
IgG2a, kappa, kappa
100μg
Store at 2-8°C. Do not freeze.
Monoclonal
Control, Flow Cytometry
eBR2a
Biotin
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
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For Research Use Only.