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Mouse IgG2a kappa, eFluor 506, clone: eBM2a, Isotype Control, eBioscience™

Mouse Isotype Control

Manufacturer:  Life Technologies LS69472480

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Catalog No. 501124963



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody.eFluor™ 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Mouse IgG2a kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2ak
IgG2a, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eBM2a
eFluor 506
Liquid
Murine
Affinity chromatography
RUO
Isotype Control
Not Applicable
Documents

For Research Use Only.