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IgG1 Rat anti-Mouse, HRP, eBioscience™

Rat Monoclonal Secondary Antibody

Manufacturer:  Life Technologies LS18401582

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Catalog No. 501128837



Description

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in western blotting, ELISA, immunohistochemical and immunocytochemical staining. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 20 μg/mL. For western blotting, this can be used at 1 μg/mL (1/500 dilution). It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications

Specifications

Mouse IgG1
Monoclonal
0.5 mg/mL
PBS with 50% glycerol and no preservative; pH 7.2
Rat
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
M1-14D12
HRP
Liquid
IgG
100 μg
4° C
Secondary
Mouse
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For Research Use Only.