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Mouse IgG1 kappa, clone: P3.6.2.8.1, Isotype Control, eBioscience™

Mouse Isotype Control

Manufacturer:  Life Technologies LS14471485

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Catalog No. 501129514



Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: This mouse IgG1 isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemistry, immunocytochemistry, immunoprecipitation and immunoblotting (WB).Applications Tested: Mouse IgG1 K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Mouse IgG1 kappa
Control
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
IgG1, IgG1k
IgG1, kappa, kappa
500μg
Store at 2-8°C.
Monoclonal
Control, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
P3.6.2.8.1
Unconjugated
Liquid
Murine
Affinity chromatography
RUO
Isotype Control
Not Applicable
Documents

For Research Use Only.