Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control. Applications Reported: The Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: This P3.6.2.8.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG1 kappa Control antibody specifically detects Mouse IgG1 kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.